MAPK14 antibody (pTyr182)

Catalog No. ABIN362314

Product Details anti-MAPK14 Antibody

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Binding Specificity: pTyr182

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of P38MAPK only when phosphorylated at tyrosine 182.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr182 (T-G-Y (p) -V-A) derived from Human P38 MAPK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-MAPK14 antibody (ABIN362314)

Su, Gu, Wang, Wang: "Lidocaine attenuates proinflammatory cytokine production induced by extracellular adenosine triphosphate in cultured rat microglia." in: Anesthesia and analgesia, Vol. 111, Issue 3, pp. 768-74, (2010) (PubMed).

Mashima, Sato, Sugimoto, Tsuruo, Seimiya: "Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions." in: Oncogene, Vol. 28, Issue 1, pp. 9-19, (2009) (PubMed).

Chen, Song, Zhou, Tong, Li, Fu, Guo, Dong, He, Qiao, Zhan, Li: "Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/beta-catenin pathway." in: Oncogene, Vol. 28, Issue 4, pp. 599-609, (2009) (PubMed).

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: P38 MAPK (MAPK14 Products)

Synonyms: CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, EXIP antibody, Mxi2 antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody, p38 antibody, p38ALPHA antibody, CRK1 antibody, Csbp1 antibody, Csbp2 antibody, Exip antibody, Hog antibody, Prkm14 antibody, Prkm15 antibody, Sapk2A antibody, p38Hog antibody, p38alpha antibody, p38b antibody, zp38b antibody, MAPK14 antibody, 186F5S antibody, BG:DS00797.3 antibody, CG7393 antibody, D-p38 antibody, D-p38 MAPK antibody, D-p38b antibody, Dm p38b antibody, Dmel\\CG7393 antibody, Dmp38b antibody, Dp38 antibody, Dp38b antibody, ESTS:186F5S antibody, Mpk34C antibody, anon-sts23 antibody, dp38b antibody, p38 MAPK antibody, p38 beta antibody, p38B antibody, p38Kb antibody, p38beta antibody, Crk1 antibody, p38-alpha antibody, p38MAPK antibody, p38a antibody, csbp antibody, mapk14a antibody, mxi2 antibody, sapk2 antibody, sapk2a antibody, AP22.98 antibody, AP22_98 antibody, ATMPK14 antibody, mitogen-activated protein kinase 14 antibody, SAPK2a antibody, MAP kinase 14A antibody, MAP kinase p38a antibody, MAPK 14A antibody, fk28c03 antibody, hm:zeh1243 antibody, wu:fk28c03 antibody, zp38a antibody, P38C-CRK antibody, mitogen-activated protein kinase 14 antibody, mitogen activated protein kinase 14 antibody, mitogen-activated protein kinase 14b antibody, p38b MAP kinase antibody, mitogen-activated protein kinase 14 S homeolog antibody, mitogen-activated protein kinase 14a antibody, CRK proto-oncogene, adaptor protein antibody, MAPK14 antibody, Mapk14 antibody, mapk14b antibody, p38b antibody, mapk14.S antibody, MPK14 antibody, mapk14a antibody, CRK antibody

Background: Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

Molecular Weight: 43 kDa

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins