HSF1 antibody (pSer303)
Quick Overview for HSF1 antibody (pSer303) (ABIN362330)
Target
See all HSF1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer303
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Specificity
- The antibody detects endogenous level of HSF1 only when phosphorylated at serine 303.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- Peptide sequence around phosphorylation site of pSer303 (P-P-S (p) -P-P) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotype
- IgG
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anti-Heat Shock Factor Protein 1 (HSF1) antibody
KD Validated HSF1 Reactivity: Human WB, FACS, IF (p) Host: Rabbit Monoclonal 23GB2715 unconjugated Recombinant Antibody
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 390
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 Reactivity: Mouse WB, ELISA, IHC, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 PE
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Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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Alternative Name
- HSF1
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Background
- DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
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Molecular Weight
- 82 kDa
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NCBI Accession
- NP_005517
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UniProt
- Q00613
Target
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