SMAD2 antibody (pThr220)

Catalog No. ABIN362418

There is 1 publication for this product available. The Rabbit Polyclonal anti-SMAD2 antibody is suitable to detect SMAD2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for SMAD2 antibody (pThr220) (ABIN362418)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD2 Antibody

Binding Specificity: pThr220

Specificity: The antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr220 (P-E-T (p) -P-P) derived from Human Smad2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-SMAD2 antibody (ABIN362418)

Zhang, Shen, Zhang, Wan, Yao, Wu, Wang, Chen, Yan, Jiang: "Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice." in: PLoS ONE, Vol. 8, Issue 3, pp. e59002, (2013) (PubMed).

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Background: Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Molecular Weight: 60 kDa

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus