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SMAD2 antibody (pThr220)
SMAD2
Reactivity: Human, Mouse, Rat
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-SMAD2 Antibody
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Target
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SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))
Binding Specificity
All epitopes for SMAD2 antibodies
pThr220
Reactivity
All reactivities for SMAD2 antibodies
Human, Mouse, Rat
Host
All hosts for SMAD2 antibodies
Rabbit
Clonality
All clonalities for SMAD2 antibodies
Polyclonal
Conjugate
All conjugates for SMAD2 antibodies
This SMAD2 antibody is un-conjugated
Application
All applications for SMAD2 antibodies
Western Blotting (WB)
Specificity
The antibody detects endogenous level of Smad2 only when phosphorylated at threonine 220.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pThr220 (P-E-T (p) -P-P) derived from Human Smad2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
References for anti-SMAD2 antibody (ABIN362418)
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Zhang, Shen, Zhang, Wan, Yao, Wu, Wang, Chen, Yan, Jiang : "Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice. " in: PLoS ONE , Vol. 8 , Issue 3 , pp. e59002 , (2013 ) (PubMed ).
Target Details for SMAD2
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Target
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))
Alternative Name
Smad2 (SMAD2 Products )
Synonyms
JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody
Background
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Molecular Weight
60 kDa
UniProt
Q15796
Pathways
Cell Division Cycle , Hormone Transport , Chromatin Binding , Protein targeting to Nucleus
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