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Paxillin antibody (AA 86-90)

Cited in 1 publication. The Rabbit Polyclonal anti-Paxillin antibody (ABIN363106) specifically detects Paxillin in WB and IF. The antibody is reactive with Human samples.
Catalog No. ABIN363106
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Quick Overview for Paxillin antibody (AA 86-90) (ABIN363106)

Target

See all Paxillin (PXN) Antibodies
Paxillin (PXN)

Reactivity

  • 243
  • 136
  • 104
  • 15
  • 7
  • 7
  • 5
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
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  • 1
Human

Host

  • 238
  • 24
  • 1
Rabbit

Clonality

  • 188
  • 75
Polyclonal

Conjugate

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  • 13
  • 9
  • 9
  • 9
  • 9
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  • 9
  • 6
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  • 4
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  • 4
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  • 4
  • 4
  • 4
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  • 3
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  • 2
  • 2
  • 1
This Paxillin antibody is un-conjugated

Application

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  • 42
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  • 32
  • 29
  • 25
  • 19
  • 14
  • 7
  • 2
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  • 1
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Western Blotting (WB), Immunofluorescence (IF)
  • Binding Specificity

    • 34
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    • 23
    • 16
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    • 6
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    • 1
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    • 1
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    AA 86-90

    Specificity

    The antibody detects endogenous level of total Paxillin protein.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen .

    Immunogen

    Peptide sequence around AA 86-90 (P-V-Y-G-S) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

    Isotype

    IgG
  • Application Notes

    Western blotting: 1:500-1:1000
    Immunofluorescence: 1:50-1:100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Wang, Bi, Ampah, Zhang, Li, Jiao, Wang, Ba, Zeng: "Lipid raft regulates the initial spreading of melanoma A375 cells by modulating ?1 integrin clustering." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 8, pp. 1679-89, (2013) (PubMed).

  • Target

    Paxillin (PXN)

    Alternative Name

    Paxillin

    Background

    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

    Molecular Weight

    68 kDa

    NCBI Accession

    NP_001074

    UniProt

    P49023

    Pathways

    MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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