p300 antibody (AA 1572-2371)

Catalog No. ABIN363282

This anti-p300 antibody is a Mouse Monoclonal antibody detecting p300 in WB, IP, ICC and IF. Suitable for Human, Mouse, Rat, Monkey and Mink.

Quick Overview for p300 antibody (AA 1572-2371) (ABIN363282)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human, Mouse, Rat, Monkey, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: RW128

Product Details anti-p300 Antibody

Binding Specificity: AA 1572-2371

Specificity: Reacts with human p300 protein (residues 1868-1921)

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Cross reacts with proteins from mouse, rat, monkey and mink.

Purification: Purified (protein A)

Immunogen: Fusion protein containing residues 1572-2371 of human p300

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF1:10-1:2000, IP1:10-1:500, WB1:250-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300

Background: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.

Gene ID: 2033

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size