HMGB1 antibody (AA 100-200)

Catalog No. ABIN363507

The Rabbit Polyclonal anti-HMGB1 antibody (ABIN363507) specifically detects HMGB1 in WB, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Sheep samples.

Quick Overview for HMGB1 antibody (AA 100-200) (ABIN363507)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 100-200

Specificity: Reacts with HMGB1 protein from Human, Mouse, Rat, Bovine, Canine, Sheep

Cross-Reactivity (Details): predicted to cross react with Equine, Rabbit, Porcine (100 % ).

Purification: Purified

Immunogen: Synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB(0.5 - 1.0 μg/mL) FC(1:100) ICC/IF(0.05 μg/mL) IHC (1:100-1:250)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.1 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Background: HMGB1 is a DAMP involved in innate immune response. It is secreted by activated dendritic cells, macrophages and necrotic cells.

Gene ID: 3146

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome