Pyruvate Dehydrogenase Kinase (AA 350-436), (C-Term) antibody

Catalog No. ABIN363520

This Rabbit Polyclonal antibody specifically detects Pyruvate Dehydrogenase Kinase in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for Pyruvate Dehydrogenase Kinase (AA 350-436), (C-Term) antibody (ABIN363520)

Target: Pyruvate Dehydrogenase Kinase

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 350-436, C-Term

Specificity: Reacts with the human PDK1

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus)

Cross-Reactivity (Details): Predicted to cross react with mouse (100 % ), rat (100 %), chicken (93 %).

Purification: Purified

Immunogen: Synthetic peptide made to a C-Terminal portion of the human PDK1 protein sequence (between residues 350-436)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ICC/IF1:1000-1:2000 WB2 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details

Target: Pyruvate Dehydrogenase Kinase

Background: Pyruvate dehydrogenase kinase isoform 1 (PDK1) inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Gene ID: 5163