Pyruvate Dehydrogenase Kinase (AA 300-400) antibody

Catalog No. ABIN363521

The Rabbit Polyclonal anti-Pyruvate Dehydrogenase Kinase antibody is suitable to detect Pyruvate Dehydrogenase Kinase in samples from Human. It has been validated for WB.

Quick Overview for Pyruvate Dehydrogenase Kinase (AA 300-400) antibody (ABIN363521)

Target: Pyruvate Dehydrogenase Kinase

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 300-400

Specificity: Reacts with the human PDK1

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Zebrafish, Chicken, Pig (Porcine)

Cross-Reactivity (Details): Predicted to cross react with mouse, rat, bovine (100 % ), chicken (92 %), porcine (91 %), primate (100 %) and zebrafish (91 %).

Purification: Purified

Immunogen: Synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
WB0.5 - 2 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details

Target: Pyruvate Dehydrogenase Kinase

Background: Pyruvate dehydrogenase kinase isoform 1 (PDK1) inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Gene ID: 5163