TLR9 antibody (AA 268-284) (FITC)

Catalog No. ABIN364233

Product Details anti-TLR9 Antibody (FITC)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Binding Specificity: AA 268-284

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry (Intracellular): 0.1-2 μg/10^6 cells, cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM Tris, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Sensitive to light.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9 (TLR9 Products)

Synonyms: CD289 antibody, toll like receptor 9 antibody, toll-like receptor 9 antibody, TLR9 antibody, Tlr9 antibody

Background: Name/Gene ID: TLR9
Family: Toll-like Receptor

Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9

Gene ID: 54106

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades