ITGB1 antibody

Catalog No. ABIN364254

This anti-ITGB1 antibody is a Mouse Monoclonal antibody detecting ITGB1 in WB, FACS, ELISA, IHC, IP, IHC (p) and IHC (fro). Suitable for Human and Monkey.

Quick Overview for ITGB1 antibody (ABIN364254)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ITGB1 Antibody

Brand: IHC-plus™

Specificity: Recognizes Human Integrin beta1 as confirmed with beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1. Species cross-reactivity: Monkey, cynomolgus monkey, rhesus monkey and weakly with porcine CD29. Does not cross-react with rat CD29.

Purification: Ammonium sulfate precipitation

Immunogen: Purified Human beta1 integrin.

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), Flo, IHC, IHC-Fr, IHC-P (10 μg/mL), IP (1 - 5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome