ITGB1 antibody

Catalog No. ABIN364255

This Mouse Monoclonal antibody specifically detects ITGB1 in WB, FACS, ELISA, IHC, IHC (p) and IHC (pfa). It exhibits reactivity toward Human and Rat.

Quick Overview for ITGB1 antibody (ABIN364255)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Clone: HUTS-4

Product Details anti-ITGB1 Antibody

Brand: IHC-plus™

Specificity: Specific for active conformation of human b1 integrins. Does not augment the affinity of beta 1 for ligands. Species cross-reactivity: rat.

Purification: Purified

Immunogen: Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.

Type of Immunogen: Cells

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-P (10 μg/mL), IHC-Pfa, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. FACS: 2.5 μg/5x10^5 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.1. No preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: ITGB1 / Integrin Beta 1 / CD29

Background: Name/Gene ID: ITGB1
Family: Integrin

Synonyms: ITGB1, CD29, CD29 antigen, Integrin beta-1, MDF2, MSK12, Integrin VLA-4 beta subunit, VLA-BETA, VLAB, Beta 1 integrin, FNRB, GPIIA, Integrin Beta 1, VLA-4 subunit beta

Gene ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome