NQO1 antibody

Catalog No. ABIN364280

The Mouse Monoclonal anti-NQO1 antibody is suitable to detect NQO1 in samples from Human and Rat. It has been validated for WB, IHC, IP and IHC (p).

Quick Overview for NQO1 antibody (ABIN364280)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NQO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AI80

Product Details anti-NQO1 Antibody

Specificity: Human & Rat NQ01. Does not react with mouse NQO1

No Cross-Reactivity: Mouse (Murine)

Purification: Protein G purified

Immunogen: Full length purified recombinant human NQO1.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (1:100), IP, WB (1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Alternative Name: NQO1

Background: Name/Gene ID: NQO1

Synonyms: NQO1, Azoreductase, DHQU, DT-diaphorase, Dioxin-inducible 1, DIA4, Diaphorase-4, NAD(P)H:quinone oxireductase, Phylloquinone reductase, QR1, Menadione reductase, NMOR1, NMORI, DTD, Quinone reductase 1

Gene ID: 1728

UniProt: P15559