Hexokinase 1 antibody (AA 1-917)

Catalog No. ABIN364333

This Mouse Monoclonal antibody specifically detects Hexokinase 1 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Hexokinase 1 antibody (AA 1-917) (ABIN364333)

Target: Hexokinase 1 (HK1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4D7

Product Details anti-Hexokinase 1 Antibody

Binding Specificity: AA 1-917

Purification: Protein G purified

Immunogen: Recombinant human Hexokinase1 (1-917aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hexokinase 1

Target: Hexokinase 1 (HK1)

Alternative Name: HK1 / Hexokinase 1

Background: Name/Gene ID: HK1
Subfamily: Hexokinase
Family: Non-protein Kinase

Synonyms: HK1, Brain form hexokinase, Hexokinase 1, Hexokinase type I, HK I, HK1-tb, HK1-tc, HKI, Hexokinase type 1, HK1-ta, HXK1, Glycolytic enzyme, Hexokinase-1, Type 1 hexokinase, Type i hexokinase

Gene ID: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effect