EPH Receptor A2 antibody (AA 559-976)

Catalog No. ABIN364396

This Mouse Monoclonal antibody specifically detects EPH Receptor A2 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for EPH Receptor A2 antibody (AA 559-976) (ABIN364396)

Target: EPH Receptor A2 (EPHA2)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3D7

Product Details anti-EPH Receptor A2 Antibody

Binding Specificity: AA 559-976

Purification: Protein G purified

Immunogen: Recombinant human EphA2 (559-976aa) purified from E. coli. NCBI Accession No.: NP_004422.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EPHA2 / EPH Receptor A2

Background: Name/Gene ID: EPHA2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA2, CTPA, EPH receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, ECK, Sek-2, ARCC2, Soluble EPHA2 variant 1, CTPP1

Gene ID: 1969

NCBI Accession: NP_004422

UniProt: P29317

Pathways: RTK Signaling