HMGA1 antibody (AA 12-23)

Catalog No. ABIN364402

This Goat Polyclonal antibody specifically detects HMGA1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog, Pig, Horse, Rabbit, Bat, Hamster and Monkey.

Quick Overview for HMGA1 antibody (AA 12-23) (ABIN364402)

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Reactivity: Human, Rat, Mouse, Dog, Pig, Horse, Rabbit, Bat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGA1 Antibody

Binding Specificity: AA 12-23

Specificity: Human HMGA1. This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Horse, Rabbit, Pig (100%) Gibbon, Bovine, Opossum (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-LASKQEKDGTEK from an internal region of human HMGA1 (NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Horse, Rabbit, Pig (100%), Gibbon, Bovine, Opossum (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Alternative Name: HMGIY / HMGA1

Background: Name/Gene ID: HMGA1

Synonyms: HMGA1, High mobility group protein A1, High mobility group protein R, HMG-R, Hmg-i, HMGIY, High mobility group AT-hook 1, HMG-I(Y), HMGA1A

Gene ID: 3159

NCBI Accession: NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910

Pathways: Nuclear Hormone Receptor Binding, Autophagy