Vimentin antibody

Catalog No. ABIN364428

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in IHC and IHC (p). Suitable for Human, Mouse, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat, Rabbit, Hamster, Horse and Gerbil.

Quick Overview for Vimentin antibody (ABIN364428)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken, Monkey, Dog, Cat, Rabbit, Hamster, Horse, Gerbil

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Vimentin Antibody

Brand: IHC-plus™

Specificity: Recognizes porcine Vimentin. Predicted species cross-reactivity: Human, monkey, bovine, equine, rabbit, canine, feline, rat, mouse, hamster, gerbil and chicken.

Purification: Protein G purified

Immunogen: Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: Sarcomas. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives, no stabilizing proteins added.

Preservative: Azide free

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis