GRB2 antibody

Catalog No. ABIN364459

This Mouse Monoclonal antibody specifically detects GRB2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow and Hamster.

Quick Overview for GRB2 antibody (ABIN364459)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human, Mouse, Rat, Cow, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GRB2 Antibody

Specificity: Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.

Purification: Protein A purified

Immunogen: Recombinant rat GRB2/Ash.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 mg/mL. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2

Background: Name/Gene ID: GRB2

Synonyms: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, EGFRBP-GRB2, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2, Grb3-3, HT027, NCKAP2

Gene ID: 2885

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation