CD40 Ligand antibody (PE)

Catalog No. ABIN370949

The Hamster Monoclonal anti-CD40 Ligand antibody is suitable to detect CD40 Ligand in samples from Mouse. It has been validated for FACS.

Quick Overview for CD40 Ligand antibody (PE) (ABIN370949)

Target: CD40 Ligand (CD40LG)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MR1

Product Details anti-CD40 Ligand Antibody (PE)

Characteristics: Synonyms: CD40 ligand, CD40-L, CD40LG, TNFSF5, TRAP, GP39, Tumor necrosis factor ligandsuperfamily member 5, TNF-related activation protein, T-cell antigen Gp39

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: < / 2 μg/10^6 cells. (Ref.1,5)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and a stabilizing agent.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L

Background: CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. (Ref.1-7) It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. (Ref.6) The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. (Ref.1,5) Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens, yet does not alter the immune response to TI antigens. (Ref.4)Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints