CTLA4 antibody

Catalog No. ABIN371061

The Hamster Monoclonal anti-CTLA4 antibody (Clone 9H10) (ABIN371061) specifically detects CTLA4 in FACS, IP and Func. The antibody is reactive with Mouse samples.

Quick Overview for CTLA4 antibody (ABIN371061)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 9H10

Product Details anti-CTLA4 Antibody

Specificity: This Monoclonal antibody is specific to CD152/CTLA-4 costimulatory receptor (Mr. 69 kDa).

Characteristics: Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Purification: Purified

Immunogen: Heat-killed Staphylococcus A bacteria coated with mouse CTLA-4/human IgG1 fusionprotein. (Ref. 1)

Isotype: IgG

Application Details

Application Notes: Flow Cytometry: < / = 3 μg/10^6 cells. Immunoprecipitation. In vitro and in vivo blocking of CTLA-4 action by soluble antibody. Promotion of in vitro CTLA-4 signalling by cross-linked antibody. See Ref.1 for more details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152 / CTLA4

Background: CD152, also known as CTLA-4, is homologous to the costimulatory molecule CD28. (Ref.1,2) Like CD28, the CD152 Molecule binds the family of B7 counter-receptors (CD80/B7-1 and CD86/B7-2) on antigen-presenting cells. (Ref.3) CTLA-4 is not expressed on resting T lymphocytes but is induced 2-3 days following T-cell stimulation via the T cell receptor (TCR) complex. (Ref.1,3,4) Whereas CD28 provides a positive costimulatory signal, CD152/CTLA-4 is a negative regulator of T-cell activation, possibly by inhibiting tyrosine kinase signalling through the TCR through its association with tyrosine phosphatases such as SHP-2. (Ref.1,5,6)Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4

Gene ID: 100505288

UniProt: P09793

Pathways: Cancer Immune Checkpoints