FADD
Reactivity: Human, Mouse, Rat, Monkey, Pig
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: 1/3,000-1/5,000Immunoprecipitation. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
FADD
(Fas (TNFRSF6)-Associated Via Death Domain (FADD))
MORT1 antibody, Mort1/FADD antibody, Mort1 antibody, TNFRSF6 antibody, adaptor antibody, fadd antibody, mort1 antibody, xfadd antibody, Fas associated via death domain antibody, Fas (TNFRSF6)-associated via death domain antibody, Fas associated via death domain S homeolog antibody, Fas (tnfrsf6)-associated via death domain antibody, FADD antibody, Fadd antibody, fadd.S antibody, fadd antibody
Background
Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1a-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation. (Ref.1-6)Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity