IRAK4 antibody (AA 38-54, AA 120-136)

Catalog No. ABIN372431

The Rabbit Polyclonal anti-IRAK4 antibody is suitable to detect IRAK4 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for IRAK4 antibody (AA 38-54, AA 120-136) (ABIN372431)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRAK4 Antibody

Binding Specificity: AA 38-54, AA 120-136

Specificity: This antibody recognizes Interleukin 1 Receptor-Associated Kinase 4 (IRAK4).

Cross-Reactivity (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse.

Purification: Protein G Chromatography.

Immunogen: A mixture of synthetic peptides containing amino acids 38-54 and 120-136 of Mouse IRAK-4.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members. The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified. IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64

Gene ID: 51135

NCBI Accession: NP_001107654

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades