SMAD2 antibody (AA 234-249)

Catalog No. ABIN372441

The Rabbit Polyclonal anti-SMAD2 antibody (ABIN372441) specifically detects SMAD2 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Chicken, Dog and Chimpanzee samples.

Quick Overview for SMAD2 antibody (AA 234-249) (ABIN372441)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMAD2 Antibody

Binding Specificity: AA 234-249

Sequence: DQQLNQSMDT GSPAEL

Specificity: This antibody recognizes SMAD, Mothers Against DPP Homolog 2 (SMAD2).

Cross-Reactivity (Details): Species reactivity (expected):Bovine (Cow), Chimpanzee, Chicken, Dog (Canine), Mouse and Rat.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to Amino acids 234-249 of human SMAD2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background: SMAD2 is an intracellular protein belonging to the Dwarfin (DWA/B)/SMAD family. SMAD2 associates with SMAD4 for translocation to nucleus. It acts as an intracellular mediator of TGFbeta family of cytokines and activin type 1 receptor (ACVR1C). Hence it regulates multiple cellular processes like cell growth, proliferation, differentiation, and apoptosis and also cooperates with transcription factors to regulate expression of defined genes. Apart from playing an important role in TGFbeta1-induced CTGF expression they also act as markers of EMT in human PTECs. It also acts as an integrator of multiple signals in the regulation of NOS3 expression. It is ubiquitously expressed in most of the tissues and is responsible for colorectal, hepatocellular, lung and breast carcinomas as well as cervical cancer.Synonyms: JV18-1, MAD homolog 2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD family member 2, SMAD-2

Gene ID: 4087

NCBI Accession: NP_001003652

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus