DDC antibody

Catalog No. ABIN372608

The Rabbit Polyclonal anti-DDC antibody is suitable to detect DDC in samples from Rat and Cow. It has been validated for WB.

Quick Overview for DDC antibody (ABIN372608)

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivity: Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDC Antibody

Specificity: Specific for the ~55k DDC protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography using an AminoLink® Plus column matrix to which purified recombinant Bovine DDC was coupled.

Immunogen: SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: AADC / DDC

Background: DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.Synonyms: Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase

Gene ID: 24311

NCBI Accession: NP_036677

UniProt: P14173

Pathways: Dopaminergic Neurogenesis