Ephrin B1 antibody (pTyr317)

Catalog No. ABIN372620

The Rabbit Polyclonal anti-Ephrin B1 antibody is suitable to detect Ephrin B1 in samples from Human, Mouse, Rat, Chicken, Cow, Zebrafish (Danio rerio) and Xenopus laevis. It has been validated for WB.

Quick Overview for Ephrin B1 antibody (pTyr317) (ABIN372620)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Mouse, Rat, Chicken, Cow, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Ephrin B1 Antibody

Binding Specificity: pTyr317

Specificity: Specific for the ~46k Ephrin-B protein phosphorylated at Tyr317. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Chicken, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken Ephrin-B.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: Ephrin-B1

Background: Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2

Gene ID: 395896

NCBI Accession: NP_990366

UniProt: O73612

Pathways: RTK Signaling