IFNAR1 antibody (pSer535, pSer539)

Catalog No. ABIN372660

This anti-IFNAR1 antibody is a Rabbit Polyclonal antibody detecting IFNAR1 in WB and IHC (fro). Suitable for Human, Mouse, Rat, Cow, Dog, Non-Human Primate and Sheep. This Primary Antibody has been cited in 1 publication.

Quick Overview for IFNAR1 antibody (pSer535, pSer539) (ABIN372660)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Non-Human Primate, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IFNAR1 Antibody

Binding Specificity: pSer535, pSer539

Specificity: This antibody is specific for the IFNAR1 protein phosphorylated at Ser535,539. Note: The Molecular Weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells, the mature form is ~110-130k.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Human, Mouse, Sheep and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser535,539 of Human IFNAR1.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-IFNAR1 antibody (ABIN372660)

Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, (2010) (PubMed).

Target Details for IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Alternative Name: IFNAR1

Background: Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).Synonyms: CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-alpha Receptor alpha, IFN-alpha/beta receptor 1, Interferon-alpha/beta receptor alpha chain, Type I interferon receptor 1

Gene ID: 3454

NCBI Accession: NP_000620

UniProt: P17181

Pathways: JAK-STAT Signaling, Hepatitis C