MAP2 antibody

Catalog No. ABIN372661

This Chicken Polyclonal antibody specifically detects MAP2 in WB, IF and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Cow and has been mentioned in 2+ publications.

Quick Overview for MAP2 antibody (ABIN372661)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Human, Mouse, Rat, Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP2 Antibody

Specificity: This antibody is specific for the ~280k MAP2 protein.

Cross-Reactivity (Details): Species reactivity (tested):Bovine, Human, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Bovine MAP2 isolated from brain by the GTP microtubule cycling method.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/20,000. Immunohistochemistry. Immunofluorescence: 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-MAP2 antibody (ABIN372661)

Klüssendorf, Song, Schau, Morellini, Dityatev, Koliwer, Kreienkamp: "The Golgi-Associated PDZ Domain Protein Gopc/PIST Is Required for Synaptic Targeting of mGluR5." in: Molecular neurobiology, Vol. 58, Issue 11, pp. 5618-5634, (2022) (PubMed).

Jacquemet, Paatero, Carisey, Padzik, Orange, Hamidi, Ivaska: "FiloQuant reveals increased filopodia density during breast cancer progression." in: The Journal of cell biology, Vol. 216, Issue 10, pp. 3387-3403, (2017) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP-2

Background: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gene ID: 4133

NCBI Accession: NP_002365

UniProt: P11137