NEFM antibody (C-Term)

Catalog No. ABIN372674

The Chicken Polyclonal anti-NEFM antibody has been validated for WB, IF and IHC (fro). It is suitable to detect NEFM in samples from Human, Rat, Mouse and Chicken.

Quick Overview for NEFM antibody (C-Term) (ABIN372674)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human, Rat, Mouse, Chicken

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NEFM Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes the ~145k Neurofilament M protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Chicken, Mouse and Rat.

Purification: Ig Fraction

Immunogen: Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Isotype: Ig Fraction

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament M (160 kD)

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Ref.1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (Ref.2).Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

Gene ID: 24588

NCBI Accession: NP_058725

UniProt: P12839

Pathways: Brown Fat Cell Differentiation