NMDAR2A antibody (N-Term)

Catalog No. ABIN372681

This anti-NMDAR2A antibody is a Rabbit Polyclonal antibody detecting NMDAR2A in WB, IF and IHC (fro). Suitable for Rat, Mouse and Cow.

Quick Overview for NMDAR2A antibody (N-Term) (ABIN372681)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NMDAR2A Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes the ~180k NR2A subunit of the NMDA Receptor.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine and Mouse.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide from the N-terminus of the NR2A subunit of rat NMDA Receptor.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Background: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A

Gene ID: 2903

NCBI Accession: NP_000824

UniProt: Q12879

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity