GRIN2C antibody (N-Term)

Catalog No. ABIN372689

The Rabbit Polyclonal anti-GRIN2C antibody is suitable to detect GRIN2C in samples from Human, Mouse and Rat. It has been validated for WB and IP.

Quick Overview for GRIN2C antibody (N-Term) (ABIN372689)

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-GRIN2C Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes the ~140k NR2C subunit of the NMDA Receptor. Also labels the ~180k NR2A and the ~180k NR2B subunits of the NMDA Receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Fusion protein from the N-terminus of the NR2C-subunit of Rat NMDA receptor.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunofluorescence: 1/1000-1/2000 (Frozen Sections). Immunohistochemitry: 1/1000-1/2000 (Frozen Sections). Immunoprecipitation: 3 μL per 200 μg lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: After reconstitution Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for GRIN2C

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Alternative Name: NMDA Receptor 2C

Background: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996).Synonyms: GRIN2C, Glutamate [NMDA] receptor subunit epsilon-3, N-methyl D-aspartate receptor subtype 2C, NMDAR2C, NR2C

Gene ID: 24411

NCBI Accession: NP_036707

UniProt: Q00961

Pathways: Synaptic Membrane