Parkin antibody (pSer378)

Catalog No. ABIN372698

The Rabbit Polyclonal anti-Parkin antibody is suitable to detect Parkin in samples from Human. It has been validated for WB.

Quick Overview for Parkin antibody (pSer378) (ABIN372698)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Parkin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Parkin Antibody

Binding Specificity: pSer378

Specificity: This antibody recognizes the ~52k Parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser378 of human Parkin.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: PARK2 / Parkin

Background: Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).Synonyms: AR-JP, E3 ubiquitin-protein ligase parkin, LPRS2, PDJ, PRKN, Parkinson disease 2, Parkinson juvenile disease protein 2

Gene ID: 5071

NCBI Accession: NP_004553

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway