Tryptophan Hydroxylase 1 antibody

Catalog No. ABIN372730

This Sheep Polyclonal antibody specifically detects Tryptophan Hydroxylase 1 in WB and IHC (fro). It exhibits reactivity toward Human and Rat.

Quick Overview for Tryptophan Hydroxylase 1 antibody (ABIN372730)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Human, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Specificity: Specific for the ~55k Tryptophan Hydroxylase protein. The antibody has been directly tested for reactivity in Western blots with Human and Rat tissue. Based upon the relatively high degree of homology of Tryptophan Hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in Rabbit tissues.

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography.

Immunogen: Recombinant rabbit Tryptophan Hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1/1,000. Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan 5-Hydroxylase 1 (TPH1)

Background: Tryptophan Hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

Gene ID: 7166

NCBI Accession: NP_004170

UniProt: P17752

Pathways: Feeding Behaviour