Tyrosine Hydroxylase antibody (pSer31)

Catalog No. ABIN372737

The Rabbit Polyclonal anti-Tyrosine Hydroxylase antibody has been validated for WB, IF and IHC (fro). It is suitable to detect Tyrosine Hydroxylase in samples from Rat, Mouse and Non-Human Primate. There are 2+ publications available.

Quick Overview for Tyrosine Hydroxylase antibody (pSer31) (ABIN372737)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat, Mouse, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer31

Specificity: Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.

Cross-Reactivity (Details): Species reactivity (expected):Non-Human Primates.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat Tyrosine Hydroxylase (TH).

Isotype: IgG

Application Details

Application Notes: Western blotting: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-Tyrosine Hydroxylase antibody (ABIN372737)

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine 3-Monooxygenase (TH)

Background: Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.Synonyms: TYH, Tyrosine 3-hydroxylase

Gene ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour