JAG1 antibody (AA 110-125)

Catalog No. ABIN372790

This Rabbit Polyclonal antibody specifically detects JAG1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat and Chimpanzee.

Quick Overview for JAG1 antibody (AA 110-125) (ABIN372790)

Target: JAG1 (Jagged 1 (JAG1))

Reactivity: Human, Mouse, Rat, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-JAG1 Antibody

Binding Specificity: AA 110-125

Specificity: Recognizes Human Jagged-1 (JAG1).

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Rat and Mouse (100 % homology).Canine (81 %) and Xenopus laevis (85 %).
Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide corresponding to aa 110-125 of Human Jagged-1 protein.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2500-1/10000. Western Blot: 1/250-1/1000. Detects a band approximately 150 kD in size corresponding toJagged-1 in Mouse liver whole cell lysate and a 70 kD band in Human brain and kidneylysates. Immunocytochemistry. Immunofluorescence: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.53 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: CD339 / JAG1

Background: Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors. JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro. Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.Synonyms: JAGL1, Jagged-1, hJ1

Gene ID: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Notch Signaling, Stem Cell Maintenance