CD20 antibody (C-Term)

Catalog No. ABIN372867

The Rabbit Polyclonal anti-CD20 antibody (ABIN372867) specifically detects CD20 in WB, IHC (p) and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for CD20 antibody (C-Term) (ABIN372867)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-CD20 Antibody

Binding Specificity: C-Term

Specificity: This antibody detetcs CD20 at C-term.
Cellular Localization: Cell membrane / cytoplasm

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide derived from C-terminus of human CD20 protein

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections (1: 100). Western blot. Immunoprecipitation. Positive Control: Tonsil or Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.6, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: CD20 is also called L26 or membrane spanning 4 domains (MS4A1). It is a 33 kd phosphoprotein with 3 hydrophobic regions that traverse the cell membrane, creating a structure similar to an ion channel that allows for the influx of calcium ions required for cell activation. Initially expressed on B cells after CD19/CD10 expression and before CD21/CD22 and surface immunoglobulin expression. It is retained on mature B cells until plasma cell development.Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Gene ID: 931

NCBI Accession: NP_068769

UniProt: P11836