IL-33 antibody (Center)

Catalog No. ABIN372914

The Rabbit Polyclonal anti-IL-33 antibody is suitable to detect IL-33 in samples from Human, Rat and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for IL-33 antibody (Center) (ABIN372914)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IL-33 Antibody

Binding Specificity: Center

Specificity: This antibody recognizes Interleukin-33 (IL33).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated corresponding to a 19 amino acid peptide from near the center of Human IL33.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: Interleukin-33 (IL33)

Background: IL33 is a cytokine which belongs to the IL-1 superfamily, and it induces helper T cells to produce type 2 cytokines. This cytokine was previously named NF-HEV 'nuclear factor (NF) in high endothelial venules' (HEVs), as it was originally identified in these specialized cells. IL33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein, activating intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-4, IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the the severe pathological changes observed in mucosal organs following administration of IL33.Synonyms: C9orf26, IL-1F11, IL-33, IL1F11, Interleukin-1 family member 11, NF-HEV, NFHEV, Nuclear factor from high endothelial venules

Gene ID: 90865

NCBI Accession: NP_001186569

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response