ITK antibody (C-Term)

Catalog No. ABIN374095

This Goat Polyclonal antibody specifically detects ITK in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for ITK antibody (C-Term) (ABIN374095)

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ITK Antibody

Binding Specificity: C-Term

Sequence: C-RLLRQLAEIA ESGL

Specificity: This antibody detects ITK/TSK at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005537.3 Genename: ITK

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 75 kDa bands seen in Jurkat Lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: Tyrosine-Protein Kinase ITK/TSK

Background: Synonyms: EMT, Kinase EMT, LYK, T-cell-specific kinase, Tyrosine-protein kinase Lyk

Gene ID: 3702

NCBI Accession: NP_005537

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway