PLK4 antibody (N-Term)

Catalog No. ABIN374175

The Goat Polyclonal anti-PLK4 antibody (ABIN374175) specifically detects PLK4 in WB. The antibody is reactive with Human, Cow and Dog samples.

Quick Overview for PLK4 antibody (N-Term) (ABIN374175)

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Reactivity: Human, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PLK4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PLK4 Antibody

Binding Specificity: N-Term

Sequence: ATCIGEKIED FKVGN

Specificity: This antibody detects SAK / STK18 at N-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_055079.3 Genename: PLK4

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 95 kDa band observed in Human Placenta lysates(calculated MW of 105 kDa according to NP_055079.2). An additional faint band of 55 kDawas consistently observed, however this band was not blocked by the immunizing peptideand it is therefore a non-specific signal. We call for caution when used for other assaysthan Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for PLK4

Target: PLK4 (Polo-Like Kinase 4 (PLK4))

Alternative Name: PLK4 / SAK / STK18

Target Type: Phage Protein

Background: Synonyms: PLK-4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase PLK4, Serine/threonine-protein kinase Sak

Gene ID: 10733

NCBI Accession: NP_055079

UniProt: O00444

Pathways: M Phase