FXYD5 antibody (C-Term)

Catalog No. ABIN374185

This Goat Polyclonal antibody specifically detects FXYD5 in WB. It exhibits reactivity toward Human.

Quick Overview for FXYD5 antibody (C-Term) (ABIN374185)

Target: FXYD5 (FXYD Domain Containing Ion Transport Regulator 5 (FXYD5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FXYD5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FXYD5 Antibody

Binding Specificity: C-Term

Sequence: GKCRQLSRLC RNHCR

Specificity: This antibody detects FXYD5 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_054883.3, NP_659003.1, NP_001158077.1 Genename: FXYD5

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 38 kDa band in Human Spleenlysate and an approx 26 kDa in Human Lung lysate after 1 μg/mL antibody staining. Thebands were successfully blocked by incubation with the immunizing peptide. Please notethat according to the literature this protein is O-glycosylated (PMID: 12672699), which mayexplain the different sizes of bands we observe given the calculated size of 19.5 kDaaccording to NP_054883 and NP_659003. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for FXYD5

Target: FXYD5 (FXYD Domain Containing Ion Transport Regulator 5 (FXYD5))

Alternative Name: Dysadherin / FXYD5

Background: Dysadherin (FXYD5) is a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD. This protein is a negative regulator of calcium-dependent cell-cell adhesion and is implicated in the biogenesis of microvilli.Synonyms: DYSAD, FXYD domain-containing ion transport regulator 5, IWU1

Gene ID: 53827

NCBI Accession: NP_054883

UniProt: Q96DB9