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PNKP antibody (C-Term)

This Goat Polyclonal antibody specifically detects PNKP in WB. It exhibits reactivity toward Human.
Catalog No. ABIN374239
$750.00
Plus shipping costs $50.00
100 μg
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Quick Overview for PNKP antibody (C-Term) (ABIN374239)

Target

See all PNKP Antibodies
PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reactivity

  • 88
  • 19
  • 17
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Host

  • 85
  • 2
  • 1
Goat

Clonality

  • 61
  • 27
Polyclonal

Conjugate

  • 35
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This PNKP antibody is un-conjugated

Application

  • 59
  • 29
  • 22
  • 17
  • 15
  • 13
  • 13
  • 13
  • 12
  • 6
  • 3
Western Blotting (WB)
  • Binding Specificity

    • 15
    • 13
    • 9
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Sequence

    EPRLGRLYCQ FSEG

    Specificity

    This antibody detects PNKP at C-term.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human

    Purification

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    Immunogen

    Peptide from C Terminus of the protein sequence according to NP_009185.2 Genename: PNKP
  • Application Notes

    Peptide ELISA: Limit dilution 1: 16000. Western blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target

    PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

    Alternative Name

    PNKP

    Background

    Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase

    Gene ID

    11284

    NCBI Accession

    NP_009185

    UniProt

    Q96T60

    Pathways

    DNA Damage Repair, Nucleotide Phosphorylation
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