CARD11 antibody (C-Term)

Catalog No. ABIN374314

This Goat Polyclonal antibody specifically detects CARD11 in WB. It exhibits reactivity toward Human, Mouse, Cow and Dog.

Quick Overview for CARD11 antibody (C-Term) (ABIN374314)

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Reactivity: Human, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD11 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CARD11 Antibody

Binding Specificity: C-Term

Sequence: C-QRKTIWVDED QL

Specificity: This antibody detects CARD11 / CARMA1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_115791.3 Genename: CARD11

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western Blot: 1-3 μg/mL. Approx 150+65 kDa bands observed in lysates of cell line Jurkat(calculated MW of 135 kDa according to NP_115791.2). A similar pattern was observed inHEK293 transiently expressing CARD11. These bands are not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CARD11

Target: CARD11 (Caspase Recruitment Domain Family, Member 11 (CARD11))

Alternative Name: CARD11 / CARMA1

Background: CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.Synonyms: CARD-containing MAGUK protein 3, Caspase recruitment domain-containing protein 11

Gene ID: 84433

NCBI Accession: NP_115791

UniProt: Q9BXL7

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, BCR Signaling