POLL antibody (C-Term)

Catalog No. ABIN374383

This Goat Polyclonal antibody specifically detects POLL in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for POLL antibody (C-Term) (ABIN374383)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLL Antibody

Binding Specificity: C-Term

Sequence: C-LGLPYREPAE RDW

Specificity: This antibody detects DNA Polymerase Lambda at C-term.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_037406.1 Genename: POLL

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave a band at approx 40 kDa in Human Liver andHepG2 extracts at 1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Small Intestine showsnuclear staining of enterocytes and of most cells in the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: DNA Polymerase lambda

Background: DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.Synonyms: DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda

Gene ID: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response