KPNA1 antibody (N-Term)

Catalog No. ABIN374391

The Goat Polyclonal anti-KPNA1 antibody (ABIN374391) specifically detects KPNA1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Pig samples.

Quick Overview for KPNA1 antibody (N-Term) (ABIN374391)

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KPNA1 Antibody

Binding Specificity: N-Term

Sequence: TTPGKENFRL KSY-C

Specificity: This antibody detects Importin subunit alpha-1 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for KPNA1

Target: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Alternative Name: KPNA1 / Importin alpha-1

Background: Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta

Gene ID: 3836

NCBI Accession: NP_002255

UniProt: P52294

Pathways: M Phase, Protein targeting to Nucleus