ACTR1B antibody (C-Term)

Catalog No. ABIN374429

The Goat Polyclonal anti-ACTR1B antibody is suitable to detect ACTR1B in samples from Human, Mouse, Rat, Dog and Cow. It has been validated for WB and IHC (p).

Quick Overview for ACTR1B antibody (C-Term) (ABIN374429)

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTR1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACTR1B Antibody

Binding Specificity: C-Term

Sequence: C-YEEDGSRAIH RKT

Specificity: This antibody recognizes ARP1 homolog B.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_005726. Genename: ACTR1B

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.5-2 μg/mL. Approx 45 kDa band observed in human heart and brain lysates(predicted MW of 45 kDa according to NP_005726). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrialcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Alternative Name: Centractin beta

Background: In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90 % amino acid identity and 96 % amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be involved in dynein-driven vesicle movement.Synonyms: ACTR1B, ARP1B, Actin-related protein 1B, CTRN2

Gene ID: 10120

NCBI Accession: NP_005726

UniProt: P42025