IRAK4 antibody (N-Term)

Catalog No. ABIN374511

The Goat Polyclonal anti-IRAK4 antibody (ABIN374511) specifically detects IRAK4 in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for IRAK4 antibody (N-Term) (ABIN374511)

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IRAK4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IRAK4 Antibody

Binding Specificity: N-Term

Sequence: NKPITPSTYV RC

Specificity: This antibody recognizes IRAK4 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_057207.2, NP_001107654.1. Genename: IRAK4

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline containing 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IRAK4

Target: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Alternative Name: IRAK4

Background: Interleukin 1 (IL1) and lipopolysaccharide (LPS) induces cellular responses through IL1 receptor (IL1R) and Toll like receptors (TLR). IL1R associated kinases (IRAK, IRAK2, and IRAKM) regulate the activation of NF-kB and MAP kinase (MAPK) by IL1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK4. Overexpression of IRAK4 activates NF-kB and MAPK pathways. IRAK4 interacts with and phosphorylates IRAK1. IRAK4 deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL1R/TLR mediated inflammatory responses and in innate immunity.Synonyms: IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64

Gene ID: 51135

NCBI Accession: NP_001107654

UniProt: Q9NWZ3

Pathways: NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades