Choline Acetyltransferase antibody (C-Term)

Catalog No. ABIN374515

This Goat Polyclonal antibody specifically detects Choline Acetyltransferase in WB. It exhibits reactivity toward Human, Dog, Pig and Chimpanzee.

Quick Overview for Choline Acetyltransferase antibody (C-Term) (ABIN374515)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Dog, Pig, Chimpanzee

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to CHAT.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Porcine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT

Background: Choline acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA in cholinergic neurons. ChAT serves as a specific marker for cholinergic neurons in both peripheral and central nervous systems. Dysfunction of cholinergic neurons underlies aspects of clinical symptoms found in neurological and psychiatric disorders such as Alzheimer's disease, Down and Rett syndromes. Choline acetyltransferase recruits choline for the synthesis of acetylcholine at preganglionic parasympathetic and sympathetic fibres. It is also present at parasympathetic postganglionic fibres.Synonyms: CHOACTase, ChAT, Choline O-acetyltransferase, Choline acetylase, EC=2.3.1.6

Gene ID: 1103

NCBI Accession: NP_001136401

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development