Acetylcholinesterase antibody (C-Term)

Catalog No. ABIN374526

The Goat Polyclonal anti-Acetylcholinesterase antibody (ABIN374526) specifically detects Acetylcholinesterase in WB and EIA. The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for Acetylcholinesterase antibody (C-Term) (ABIN374526)

Target: Acetylcholinesterase (AChE)

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Acetylcholinesterase Antibody

Binding Specificity: C-Term

Sequence: QFDHYSKQDR CSDL

Specificity: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000656.1.

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase

Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase

Gene ID: 43, 9606

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development