HNF4A antibody (N-Term)

Catalog No. ABIN374541

The Goat Polyclonal anti-HNF4A antibody (ABIN374541) specifically detects HNF4A in WB. The antibody is reactive with Human samples.

Quick Overview for HNF4A antibody (N-Term) (ABIN374541)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HNF4A Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to HNF-4-alpha. This antibody is expected to recognise the reported isoforms a, b and c.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Porcine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4 alpha / TCF14

Background: The protein encoded by the HNF4 gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non insulin dependent diabetes mellitus type I. At least three different transcript variants encoding three different isoforms have been found for this gene.Synonyms: HNF4, HNF4A, Hepatocyte nuclear factor 4-alpha, NR2A1, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4

Gene ID: 3172

NCBI Accession: NP_000448

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process