CBX3 antibody (Internal Region)

Catalog No. ABIN374619

The Goat Polyclonal anti-CBX3 antibody (ABIN374619) specifically detects CBX3 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for CBX3 antibody (Internal Region) (ABIN374619)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBX3 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to Chromobox protein homolog 3. No cross-reactivity expected with HP1-alpha and HP1-beta.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1.0 μg/mL. Immunohistochemistry on paraffin sections: 2 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: CBX3

Background: HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.Synonyms: Chromobox protein homolog 3, HECH, HP1 gamma, HP1-gamma, HP1Hs-gamma, Heterochromatin protein 1 homolog gamma, Modifier 2 protein

Gene ID: 11335, 9606

UniProt: Q13185