Leptin Receptor antibody (Internal Region)

Catalog No. ABIN374689

This Goat Polyclonal antibody specifically detects Leptin Receptor in WB and EIA. It exhibits reactivity toward Human and Dog.

Quick Overview for Leptin Receptor antibody (Internal Region) (ABIN374689)

Target: Leptin Receptor (LEPR)

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Leptin Receptor Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognise all three reported isoforms (NP_002294.2, NP_001003679.1, NP_001003680.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-TQDDIEKHQSDAG, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: CD295 / Leptin Receptor

Background: Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.Synonyms: DB, HuB219, LEP-R, LEPR, OB receptor, OB-R, OBR

Gene ID: 3953, 9606

UniProt: P48357

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour